Partial Gene Duplication And The Formation Of Novel Genes

Partial Gene Duplication and the Formation of Novel Genes PDF
Author: Macarena Toll-Riera
Publisher:
ISBN:
Size: 42.86 MB
Format: PDF, Docs
Category : Medicine
Languages : en
Pages :
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Partial Gene Duplication And The Formation Of Novel Genes

by Macarena Toll-Riera, Partial Gene Duplication And The Formation Of Novel Genes Books available in PDF, EPUB, Mobi Format. Download Partial Gene Duplication And The Formation Of Novel Genes books, Partial Gene Duplication and the Formation of Novel Genes.



Gene Duplication And Reproductive Incompatibility In Caenorhabditis Elegans

Gene Duplication and Reproductive Incompatibility in Caenorhabditis Elegans PDF
Author: Vaishali Katju
Publisher:
ISBN:
Size: 78.26 MB
Format: PDF, ePub
Category :
Languages : en
Pages : 348
View: 4700

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Gene Duplication And Reproductive Incompatibility In Caenorhabditis Elegans

by Vaishali Katju, Gene Duplication And Reproductive Incompatibility In Caenorhabditis Elegans Books available in PDF, EPUB, Mobi Format. Download Gene Duplication And Reproductive Incompatibility In Caenorhabditis Elegans books,



Gene Duplication

Gene Duplication PDF
Author: Felix Friedberg
Publisher: BoD – Books on Demand
ISBN: 953307387X
Size: 35.82 MB
Format: PDF, ePub
Category : Medical
Languages : en
Pages : 414
View: 839

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Gene Duplication

by Felix Friedberg, Gene Duplication Books available in PDF, EPUB, Mobi Format. Download Gene Duplication books, The book Gene Duplication consists of 21 chapters divided in 3 parts: General Aspects, A Look at Some Gene Families and Examining Bundles of Genes. The importance of the study of Gene Duplication stems from the realization that the dynamic process of duplication is the "sine qua non" underlying the evolution of all living matter. Genes may be altered before or after the duplication process thereby undergoing neofunctionalization, thus creating in time new organisms which populate the Earth.



A Novel System For Detection Of Dna Double Strand Breaks And Repair In Human Cells

A Novel System for Detection of DNA Double Strand Breaks and Repair in Human Cells PDF
Author: Todd Warren Lewis
Publisher:
ISBN:
Size: 29.87 MB
Format: PDF, ePub
Category : Biology
Languages : en
Pages : 117
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A Novel System For Detection Of Dna Double Strand Breaks And Repair In Human Cells

by Todd Warren Lewis, A Novel System For Detection Of Dna Double Strand Breaks And Repair In Human Cells Books available in PDF, EPUB, Mobi Format. Download A Novel System For Detection Of Dna Double Strand Breaks And Repair In Human Cells books, Imperative to genomic stability is the ability of the cell to repair damaged DNA which can occur from numerous endogenous byproducts of metabolism or exogenous components from the environment. The Fanconi anemia pathway is a DNA repair mechanism used by human cells to resolve multiple forms of DNA damage including interstrand crosslinks (ICL). Fanconi anemia (FA) is an autosomal recessive inherited disorder characterized by genome instability, developmental abnormalities, cancer predisposition and bone marrow failure. FA is attributed to a mutations in at least 18 genes (FANCA-FANCT) that play a concerted role in DNA repair. FANCT is the latest discovery in the FA pathway and is a UBE2T ubiquitin conjugase. An FA patient with biallelic germline mutations in FANCT presented with the classical symptoms of FA with the exception of hematopoietic indications. Both mutations, a maternal duplication and a paternal deletion of exons 2-6, were AluY mediated. Upon further evaluation it was determined that the FA patient had a reversion of the maternal duplication back to a WT allele which restored FANCT function in the hematopoietic lineage. The genomic reversion was also attributed to an Alu mediated recombination (AMR) event. The factors that led to the AMR event were unknown and provided reason to investigate further. The promise of harnessing the mechanism to utilize a genomic duplication reversion could be of importance for therapeutic interventions.No current human cell line models were sufficient to address what factors are involved in the FANCT reversion, so a novel Dual Fluorescence (DF) model system was created in a HeLa cell line to test the hypothesis that Alu mediated homology directed repair is sufficient to explain genetic reversion of a partially duplicated FANCT locus. The DF model system emulates elements of the FANCT locus using partial intronic sequences and Alu elements found in the patient{u2019}s duplicated FANCT locus. Key to the model system design is the incorporation of TOM and eGFP fluorescent marker genes and an I-Sce1 recognition site into the ectopic locus. I-Sce1 is a rare endonuclease found in yeast, with no known recognition site found in humans and when expressed in the DF cells will cause a targeted DNA double strand break. The fluorescent marker genes are separated and flanked by Alu elements. After an I-Sce1 induced DSB, it is possible that AMR will occur and eliminate one of the fluorescent marker genes, thereby shifting fluorescence of the cell. This shift in fluorescence can be detected by microscopy or flow cytometry. To test whether Alu mediated homology directed repair is sufficient to explain genetic reversion of a partially duplicated FANCT locus, multiple DF cell lines were created that vary in; location of the I-Sce1 recognition site, number of Alu elements and the presence of repetitive DNA sequences. Flow cytometry of the DF cells after an I-Sce1 mediated DSB, reveals that recombination of the proximal Alu elements is the most likely repair outcome. Further evaluation of DF cell line recombinants by Flow Assisted Cell Sorting (FACS) and DNA sequencing disclose that the model system can differentiate between homology and non-homology directed repair. To test if Alu mediated homology directed repair can facilitate a genetic reversion, canonical DNA repair proteins in homology directed repair (HDR) and non-HDR pathways were impaired. The results of this experiment provide evidence that only HDR pathways support the genetic reversion. Upon evaluation of a FANCT null cell line in the DF model and the well-established U2-OS GFP model, both models indicate FANCT is involved in homology directed repair. The unique DF model system was able to determine that FANCT is also involved in non-HDR. These experiments provides evidence for the novel finding of FANT{u2019}s role in HDR and non-HDR outside of ICL resolution via the FA pathway. Another aspect to examine when assessing AMR causality is what factors may lead to the initial DNA break occurrence. There is evidence in multiple diseases that an AMR event was caused by a DNA break at a microsatellite sequence. To address the question of how repetitive DNA sequences influence Alu mediated homology directed repair, the DF cell lines were modified to include multiple microsatellite motifs. The results of an I-Sce1 induced break juxtaposed to a microsatellite sequence reveal that both homology and non-homology directed recombination events are significantly impaired, presumably by the formation of DNA secondary structure. The results of this project suggest that Alu mediated homology directed repair is sufficient to explain genetic reversion of a partially duplicated FANCT locus. Additionally, the development of the DF cell lines provide a novel model system for detection of DNA double strand breaks and repair in human cells.



Evolution Through Genetic Exchange

Evolution Through Genetic Exchange PDF
Author: Michael L Arnold
Publisher: Oxford University Press
ISBN: 0198570066
Size: 21.99 MB
Format: PDF, ePub
Category : Science
Languages : en
Pages : 252
View: 4382

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Evolution Through Genetic Exchange

by Michael L Arnold, Evolution Through Genetic Exchange Books available in PDF, EPUB, Mobi Format. Download Evolution Through Genetic Exchange books, More and more data indicate that evolution has resulted in lineages consisting of mosaics of genes derived from different ancestors. It is therefore becoming increasingly clear that the tree is an inadequate metaphor of evolutionary change. In this book, Arnold promotes the 'web-of-life' metaphor as a more appropriate representation of evolutionary change in all lifeforms.



Protein Evolution

Protein Evolution PDF
Author: Laszlo Patthy
Publisher: John Wiley & Sons
ISBN: 1444308882
Size: 64.13 MB
Format: PDF, Mobi
Category : Science
Languages : en
Pages : 392
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Protein Evolution

by Laszlo Patthy, Protein Evolution Books available in PDF, EPUB, Mobi Format. Download Protein Evolution books, This book provides an up-to-date summary of the principles of protein evolution and discusses both the methods available to analyze the evolutionary history of proteins as well as those for predicting their structure-function relationships. Includes a significantly expanded chapter on genome evolution to cover genomes of model organisms sequenced since the completion of the first edition, and organelle genome evolution Retains its reader-friendly, accessible style and organization Contains an updated glossary and new references, including a list of online reference sites



Genome Evolution

Genome Evolution PDF
Author: Axel Meyer
Publisher: Springer Science & Business Media
ISBN: 9401002630
Size: 68.82 MB
Format: PDF
Category : Science
Languages : en
Pages : 237
View: 4784

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Genome Evolution

by Axel Meyer, Genome Evolution Books available in PDF, EPUB, Mobi Format. Download Genome Evolution books, In the years since the publication of Susumu Ohno's 1970 landmark book Evolution by gene duplication tremendous advances have been made in molecular biology and especially in genomics. Studies of genome structure and function prerequisite to testing hypotheses of genome evolution were all but impossible until recent methodological advances. This book evaluates newly generated empirical evidence as it pertains to theories of genomic evolutionary patterns and processes. Tests of hypotheses using analyses of complete genomes, interpreted in a phylogenetic context, provide evidence regarding the relative importance of gene duplication. The alternative explanation is that the evolution of regulatory elements that control the expression of and interactions among genes has been a more important force in shaping evolutionary innovation. This collection of papers will be of interest to all academic and industry researchers working in the fields of molecular biology, biotechnology, genomics and genome centers.



Bioinformatics

Bioinformatics PDF
Author: Frédéric Dardel
Publisher: John Wiley & Sons
ISBN: 9780470020029
Size: 49.73 MB
Format: PDF
Category : Science
Languages : en
Pages : 252
View: 3308

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Bioinformatics

by Frédéric Dardel, Bioinformatics Books available in PDF, EPUB, Mobi Format. Download Bioinformatics books, This book is an excellent introductory text describing the use of bioinformatics to analyze genomic and post-genomic data. It has been translated from the original popular French edition, which was based on a course taught at the well-respected École Polytechnique in Palaiseau. This edition has been fully revised and updated by the authors. After a brief introduction to gene structure and sequence determination, it describes the techniques used to identify genes, their protein-coding sequences and regulatory regions. The book discusses the methodology of comparative genomics, using information from different organisms to deduce information about unknown sequences. There is a comprehensive chapter on structure prediction, covering both RNA and protein. Finally, the book describes the complex networks of RNA and protein that exist within the cell and their interactions, ending with a discussion of the simulation approaches that can be used to model these networks. Praise from the reviews: “In context of the new developments the genomic era has brought, Bioinformatics: Genomics and Post-Genomics becomes a fundamental and indispensable resource for undergraduate and early graduate students...insightfully authored...will immensely help students...in establishing important foundations while shaping their careers.” NEWSLETTER, BRITISH SOCIETY OF CELL BIOLOGY



Genetics Abstracts

Genetics Abstracts PDF
Author:
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ISBN:
Size: 38.68 MB
Format: PDF, ePub, Mobi
Category : Genetics
Languages : en
Pages :
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Genetics Abstracts

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Cumulated Index Medicus

Cumulated Index Medicus PDF
Author:
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ISBN:
Size: 65.26 MB
Format: PDF, ePub, Mobi
Category : Medicine
Languages : en
Pages :
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Cumulated Index Medicus

by , Cumulated Index Medicus Books available in PDF, EPUB, Mobi Format. Download Cumulated Index Medicus books,